Department of Oral Biology

     Oral Biology 578
           Recombinant DNA Techniques   

Course Instructor
Dr. Richard Presland 

     Oral Biology 579
            Molecular Biology & Oral Health  Applications

Course Instructors
 Dr. Richard Presland
Dr. Frank Roberts, Periodontics
Dr. Whasun Chung

ORALB 578 (offered every other year
even years)
Course Announcement
Class Schedule
Southern Blot
Grading and Examination

ORALB 579

2007 LECTURES

Lectures 1 & 2

Lecture 3

• DNA cloning animation

Lecture 4 (Dr. W. Chung)

• PCR animation
• Nature article by E. Foster et al. (1998) on Thomas Jefferson and Sally Hemmings
• Accompanying Nature News and Views article by Lander and Ellis
• Description of Real Time (Q) PCR

Lecture 5

• Gene expression profiling animation
• RNAinterference (RNAi) animation
• RNAi on NOVA
  

  This PBS site contains information about RNA interference (RNAi) and also has information on current prospective therapies being developed using this unique genetic technology for diseases such as macular degeneration. You can also watch the actual video segment from NOVA.

Lecture 6

• Slavkin article (2001) on the Human Genome Project

• Human Genome Program, U.S. DOE
• NHGRI, National Institutes of Health

Lecture 7

• Review article by Presland and Jurevic (2002)

Lecture 8

• Oral cancer article (Nagata et al., 2003)
• The Oral Cancer Foundation

HIV infection and chemokine receptor polymorphisms

1. Dean M, Carrington M, Winkler C, Huttley GA, Smith MW, Allikmets R, Goedert JJ, Buchbinder SP, Vittinghoff E, Gomperts E, Donfield S, Vlahov D, Kaslow R, Saah A, Rinaldo C, Detels R, O'Brien SJ. Genetic restriction of HIV-1 infection and pregression to AIDS by a deletion allele of the CKR5 structural gene. Hemophilia Growth and Development Study, Multicenter AIDS Cohort Study, Multicenter Hemophilia Cohort Study, San Francisco City Cohort, ALIVE Study. Science 273:1856-62, 1996.
   
   Comment in: Science 273:1797-8, 1996.
   
   This is the first description of chemokine polymorphisms that affect HIV progression (not available on-line, but can be read in the Health Sciences Library).
2. O'Brien SJ, Nelson GW. Human genes that limit AIDS. Nat. Genet. 36:565-74, 2004.
   (PubMed)
3. O'Brien SJ, Moore JP. The effect of genetic variation in chemokines and their receptors on HIV transmission and progression to AIDS. Immunol. Rev. 177:99-111, 2000
   (PubMed)

The last two reviews discuss genetic polymorphisms that affect HIV progression, including the CCR5-delta32 allele.

Lecture 9

• Review by Mostowska et al. (2003) on tooth agenesis-associated genes
• Papillon-Lefevre syndrome, OMIM link

Lecture 10 (Dr. Frank Roberts)

Lecture 11 (Dr. Frank Roberts)

USEFUL ANIMATIONS AND VIDEOS

1. Biology Animation Library
   

   This site, developed by the Dolan DNA Learning Center at Cold Spring Harbor Laboratory, has animations and discussion of restriction enzymes, the PCR, the Southern blot technique, DNA chips, (microarrays), and model organisms.  It also has a flyover (flyby) of Human Chromosome 11 which reviews chromosome structure down to the gene/DNA level.

2. Explore a Stretch of Code
   

   This PBS site allows one to examine various facets of a gene and DNA sequence.

3. Genetic Science Learning Center
   

   This site has information on many topics relevant to this course, including DNA structure and function and genetic disease.  Topics such as stem cells and gene therapy (that are only briefly covered in this course) are quite extensively discussed at this web site.