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Richard Presland, Ph.D.

Associate Professor & Graduate Program Director, Oral Biology
Adjunct Research Associate Professor, Department of Medicine
B-147
B-137
357132
rp@u.washington.edu
Community of Science Profile
Interests

Epithelial tissues, such as those that line the oral cavity and skin, function to protect animals from physical, chemical, and microbial attack, and dessication. They are essential for survival. During keratinocyte differentiation, living cells undergo desquamation to form the dead, cornified cells at the epithelial surface. These "squames" comprise a major part of the epithelial barrier and are continuously being renewed. Our research focuses on several genes that function in this process, including profilaggrin and caspase-14. Profilaggrin is a fused S100 calcium-binding protein that is the precursor of filaggrin. Caspase-14 is a member of the caspase family of cysteine endoproteases that is expressed mostly in epithelial tissues. Profilaggrin mutations are associated with the human skin disease ichthyosis vulgaris, and with the more common disorder atopic dermatitis ('eczema').

We are currently using a variety of molecular and cellular approaches to study the function of the profilaggrin N-terminus in keratinocyte differentiation. These approaches include the yeast two-hybrid assay to examine protein-protein interactions in vivo, as well as gene expression in bacteria and cultured mammalian cells to study protein-protein interactions and protein function. Recent studies have shown that the profilaggrin N-terminus associates with a number of different keratinocyte proteins, including some genes/proteins not previously associated with keratinocyte differentiation. We are further characterizing some of these genes, in particular those that have a nuclear localization and a possible role in gene regulation.

Reviews

Presland RB. Function of filaggrin and caspase-14 in formation and maintenance of the epithelial barrier. Dermatol. Sinica 27:1-14, 2009. http://www.dental.washington.edu/ob/Download/Files/People/Presland/Review%20Derm%20Sinica.pdf

Presland RB, Jurevic RJ. Making sense of the epithelial barrier: what molecular biology and genetics tell us about the functions of oral mucosal and epidermal tissues. J. Dent. Educ. 66:564-574, 2002.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12014572/

Presland RB, Dale BA. Epithelial structural proteins of skin and oral cavity: function in health and disease. Crit. Rev. Oral Biol. Med. 11: 383-408, 2000. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=11132762&query_hl=13&itool=pubmed_docsum/

Training

Dr. Presland received a B.Sc. (Hons) (1979) and a M.Sc. With Honors (1981) in Biochemistry from the University of Otago, Dunedin, New Zealand. He received a Ph.D. (1987) in Biochemistry and Molecular Biology from the University of Adelaide, South Australia. He has been at the University of Washington since 1989 and a faculty member since 1994.

Students
Courses
  1. ORALB 580 - Introduction to Molecular Biology Laboratory
    Website
  2. ORALB 579 - Molecular Biology
    Website
Publications

  1. Fatherazi S, Matsa-Dunn D, Foster BL, Rutherford RB, Somerman MJ, Presland RB. Phosphate regulates osteopontin gene transcription. J. Dent. Res. 88:39-44, 2009.

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  2. Fallon PG, Sasaki T, Sandilands A, Campbell LE, Saunders SP, Mangan NE, Callanan JJ, Kawasaki H, Shiohama A, Kubo A, Sundberg JP, Presland RB, Fleckman P, Shimizu N, Kudoh J, Irvine AD, Amagai M, McLean WH. A homozygous frameshift mutation in the mouse Flg gene facilitates enhanced percutaneous allergen priming. Nat. Genet. 41:602-8, 2009.

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  3. Denecker G, Hoste E, Gilbert B, Hochepied T, Ovaere P, Lippens S, Van den Broecke C, Van Damme P, D'Herde K, Hachem JP, Borgonie G, Presland RB, Schoonjans L, Libert C, Vandekerckhove J, Gevaert K, Vandenabeele P, Declercq W. Caspase-14 protects against epidermal UVB photodamage and water loss. Nat Cell Biol.

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  4. Fatherazi S, Presland RB, Belton CM, Goodwin P, Al-Qutub M, Trbic Z, Macdonald G, Schubert MM, Izutsu KT. Evidence that TRPC4 supports the calcium selective I(CRAC)-like current in human gingival keratinocytes. Pflugers Arch. 453:879-889, 2007.

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  5. Park K, Kuechle MK, Choe Y, Craik CS, Lawrence OT, Presland RB. Expression and characterization of constitutively active human caspase-14. Biochem. Biophys. Res. Comm. 347:941-948, 2006.

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  6. Smith FJ, Irvine AD, Terron-Kwiatkowski A, Sandilands A, Campbell LE, Zhao Y,Liao H, Evans AT, Goudie DR, Lewis-Jones S, Arseculeratne G, Munro CS, Sergeant A, O'regan G, Bale SJ, Compton JG, Digiovanna JJ, Presland RB, Fleckman P, McLean WH. Loss-of-function mutations in the gene encoding filaggrin cause ichthyosis vulgaris. Nat Genet. 38:337-342, 2006.

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  7. Cai S, Fatherazi S, Presland RB, Belton CM, Roberts FA, Goodwin PC, Schubert MM, Izutsu KT. Evidence that TRPC1 contributes to calcium-induced differentiation of human keratinocytes. Pflugers Archives, Eur. J. Physiol. 452:43-52, 2006.

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  8. Wang H, Presland RB, Piepkorn M. A search for CDKN2A/p16INK4a mutations in melanocytic nevi from patients with melanoma and spouse controls by use of laser-captured microdissection. Arch. Dermatol. 141:177-180, 2005.

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  9. Presland RB, Fleckman P. Tetracycline-regulated gene expression in epidermal keratinocytes. Methods Mol. Biol. 289:273-286, 2005.

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  10. Presland RB, Coulombe PA, Eckert RL, Mao-Qiang M, Feingold KR, Elias PM. Barrier function in transgenic mice overexpressing K16, involucrin, and filaggrin in the suprabasal epidermis. J. Invest. Dermatol. 123:603-606, 2004.

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  11. Chien AJ, Presland RB, Kuechle MK. Processing of native caspase-14 occurs at an atypical cleavage site in normal epidermal differentiation. Biochem Biophys Res Commun. 296:911-917, 2002.

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  12. Pearton DJ, Dale BA, Presland RB. Functional analysis of the profilaggrin N-terminal peptide. Identification of peptide sequences that regulate cytoplasmic and nuclear distribution. J. Invest. Dermatol. 119:661-669, 2002.

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  13. Presland RB, Kuechle MK, Lewis SP, Fleckman P, Dale BA. Regulated expression of human filaggrin in keratinocytes results in cytoskeletal disruption, loss of cell-cell adhesion, and cell cycle arrest. Exp. Cell Res. 270:199-213, 2001.

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  14. Pearton DJ, Nirunsuksiri W, Rehemtulla A, Lewis SP, Presland RB, Dale BA. Proprotein convertases expression and localization in epidermis: evidence for multiple roles and substrates. Exp. Dermatol. 10:193-203, 2001.

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  15. Kuechle MK, Predd HM, Fleckman P, Dale BA, Presland RB. Caspase 14, a keratinocyte specific caspase: mRNA splice variants and expression pattern in embryonic and adult mouse. Cell Death Diff. 8:868-870, 2001.

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  16. Presland RB, Boggess D, Lewis SP, Hull C, Fleckman P, Sundberg JP. Loss of normal profilaggrin and filaggrin in flaky tail (ft/ft) mice: an animal model for the filaggrin-deficient skin disease ichthyosis vulgaris. J. Invest. Dermatol. 115:1072-1081, 2000.

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